Bioprotocols

Transgenic Mice

Transgenic Mice

Transgenic Mice

Omar giving a talk at Hunter college; sorry for the poor quaility bc iSight was not behaving as usual.

http://eppendorfna.com/CellTech The technique of introducing new genetic material into the germline of mammals has been a major development in biotechnology over the last decades. \"Transgenic animals\" are animals whose chromosomes contain stable, integrated copies of exogenous genes, additional copies of endogenous genes or gene constructs. They are frequently created by two different techniques: microinjection of DNA into the pronucleus of zygotes and injection of embryonic stem cells into blastocysts. This video covers microinjection of DNA into the pronucleus of zygotes. [More] [Less]

This video describes the use of transgenic organisms to study evolutionarily conserved patterns of development and function. HHMI description: What do humans, flies, and worms have in common? More than you might think. See how transgenic organisms are engineered, and how they enable researchers to study genetic diseases. Also featured on the HHMI DVD, Learning from Patients: The Science of Medicine, available free from HHMI. [More] [Less]

This video describes the use of transgenic organisms to study evolutionarily conserved patterns of development and function. HHMI description: What do humans, flies, and worms have in common? More than you might think. See how transgenic organisms are engineered, and how they enable researchers to study genetic diseases. Also featured on the HHMI DVD, Learning from Patients: The Science of Medicine, available free from HHMI. [More] [Less]

Excerpts of videos I shot and edited for the Journal of Visualized Experiments (www.jove.com).

Nuclear Transfer is a form of cloning. The steps involve removing the DNA from an oocyte and while(unfertilized egg), and injecting the nucleus which contains the DNA to be cloned. In rare instances, the newly constructed cell will divide normally, replicating the new DNA while remaining in a pluripotent state. If the cloned cells are placed in the uterus of a female mammal, a cloned organism develops to term in rare instances. This is how Dolly the Sheep and many other species were cloned. Alternatively, if cells are extracted from the cloned cells during very early embryonic stages (blastocyst or morula), embryonic stem cells can be created. These cells can be grown in laboratories indefinitely and can theoretically be made into any of the 200+ cell types in the mammalian body, and thus are an extraordinary tool for biologists as well as a therapeutic agent with the potential to treat currently untreatable medical conditions. [More] [Less]

Animal cloning gone bad, waaaay bad Check out our website: www.KnGproduction.com

If you\'ve ever wondered how to clone a sheep, this simple step-by-step demonstration is just what you\'ve been looking for. Ten simple steps is all it takes to go from egg cell to infant sheep clone. DO NOT ATTEMPT AT HOME.

Dr Andrew Corbett presents the Stem Cell and Human Cloning Debate from an ethical perspective...

cloning video i made for class my junior year of high school

A 10 minute discussion on the difference between embryonic and adult stem cells. Focus is on the use of mesenchymal stem cells.

Compilation of \"true\" to size, shape and speed animations provided by the Howard Hughes Medical Institute showing each part of the Central Dogma of Molecular Biology using the Human globin gene as an example--all in one video

This lecture by Dr. Nadia Rosenthal discusses the importance of adult stem cells in the tissue maintenance, development and regeneration. Part 2 of 6. HHMI description: Mature organisms have stem cells of various sorts, called adult stem cells. Adult stem cells supply cells that compensate for the loss of cells from normal cell death and turnover, such as the ever-dying cells of our skin, our blood, and the lining of our gut. They are also an essential source of cells for healing and regeneration in response to injury. Some animals, such as sea stars, newts, and flatworms, are capable of dramatic feats of regeneration, producing replacement limbs, eyes, or most of a body. It is an evolutionary puzzle why mammals have more limited powers of regeneration. Researchers are interested in pinpointing where adult stem cells reside and in understanding how flexible adult stem cells are in their ability to produce divergent cells such as muscle and red blood cells. Understanding the sources and the rules for the differentiation of adult stem cells is essential for tapping their therapeutic potential. Since consenting adults can provide adult stem cells, some people think that adult stem cells may be a less controversial area of research than embryonic stem cells. [More] [Less]

Nuclear Transfer is a form of cloning. The steps involve removing the DNA from an oocyte and while(unfertilized egg), and injecting the nucleus which contains the DNA to be cloned. In rare instances, the newly constructed cell will divide normally, replicating the new DNA while remaining in a pluripotent state. If the cloned cells are placed in the uterus of a female mammal, a cloned organism develops to term in rare instances. This is how Dolly the Sheep and many other species were cloned. Alternatively, if cells are extracted from the cloned cells during very early embryonic stages (blastocyst or morula), embryonic stem cells can be created. These cells can be grown in laboratories indefinitely and can theoretically be made into any of the 200+ cell types in the mammalian body, and thus are an extraordinary tool for biologists as well as a therapeutic agent with the potential to treat currently untreatable medical conditions. [More] [Less]