Biotechniques

Your Lab's Handbook

Saturday
May 19th
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Home Biochemistry HPLC Troubleshooting

HPLC Troubleshooting

Troubleshooting High Performance Liquid Chromatography (from ASRG)

1. Peaks

Broad peaks

Ghost peaks

Negative peaks

Peak doubling

Peak fronting

Peak tailing

Spikes

2. Leaks

Leak at column or fittings

Leak at detector

Leak at injection valve

Leak at pump