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1) Transfer to a 24 wells plate the desired colonies.
2) Once the cells are attached (at least 8 hours after tripsinizing them) add ~1 ml of
DME (met-, cys-).
3) Add 3l of Met 35S, Cys 35S trans label.
4) Incubate at least 5 hours at 370C (Put plate(s) inside a tupperware, containing a
beaker with activated charcoal, and close loosely).
5) Collect the media into 1.7 mL tubes and add 5l of the desired antibody.
6) Incubate at least 1 hour using the mixer located at cold room.
7) Centrifuge briefly.
8) Add 100l of a 75:25 protein A:50mM Tris mixture.
9) Incubate at least 1 hour using the mixer located at cold room.