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Preparation of bone marraow macrophages from mice.
(source: openwetware)
Procedure
Day 0
Dissect out mouse femurs and tibias (total of four bones per mouse).
Flush the bone marrow from each bone with 5-10 cc of BM20 using a syringe and a 26G needle. Combine the bone marrow from a single mouse into a 50-mL conical tube. Vortex briefly, and bring total volume to 50mL.
Plate cells into non-TC treated 10-cm petri dishes (Fisherbrand Cat #08-757-12), 10mL per Petri dish, 5 Petri dishes/mouse
Incubate at 37°C/5% CO2.