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Pulmonary Epithelial Cell Culture
Author(s): Ben Forbes
Pub. Date: Mar-08-2002; DOI:10.1385/1-59259-185-X:65
Summary: type I cell-like monolayers with high transepithelial electrical resistances, which are suitable to study alveolar permeability and drug transport (7). The isolation of alveolar type II cells from...
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Purification of Rat Pancreatic ß-Cells by Fluorescence-Activated Cell Sorting
Author(s): Geert Stangé, Mark Casteele, Harry Heimberg
Pub. Date: Feb-04-2003; DOI:10.1385/1-59259-377-1:015
Summary: with anatomically associated acinar cells is inevitable during isolation and may have a major influence on the specificity of experiments. 1 Introduction The β-cell is receptive to intricate hormonal...
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The Derivation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation
Author(s): Edward J. Gallagher, Jim McWhir
Pub. Date: Mar-24-2004; DOI:10.1385/1-59259-741-6:099
Summary: frequency to ES lines from the permissive 129 strain. We concluded that the removal of differentiating lineages relaxes inhibition of the stem cell population, permitting the isolation of ES cells from...
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Author(s): Hanneke Schuitemaker, Neeltje A. Kootstra
Pub. Date: May-17-2005; DOI:10.1385/1-59259-907-9:017
Summary: for 2 to 3 d, starting at a cell density of 5 × 106/mL. PHA-stimulated cells are then centrifuged at 425g and resuspended in IL-2 medium at a cell density of 1 × 106/mL. 3.3.1. Isolation of Bulk HIV...
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Author(s): Helmut Jonuleit, Edgar Schmitt
Pub. Date: Nov-30-2004; DOI:10.1385/1-59259-862-5:285
Summary: ). 16. Cell strainer, 70 µm, nylon (Falcon). 17. MACS Separator (Miltenyi). 18. Magnetic Particle Concentrator for Dynabeads (Dynal). 19. Sample mixer (MX1, Dynal). 2.1 Isolation and Testing of Human...
Abstract | Full Text | PDF (282K)