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Procedure:
1) Dissolve/dilute coating substrate in ddH2O at 4 C. A common working dilution for the laminin-1 positive control and BSA (Sigma A8412) negative control is 40 ug/ml. For SN-peptide,
use 20 uM (MW of SN-peptide is 2412) for plateau or 2.5 - 5 uM for half-maximal adhesion. For fragment E8 or laminin-1 plateau and half-maximal adhesion are usually achieved at 0.5 and 0.05 uM, respectively.
2) Add coating solution (100 ul/well) with multipipetter to wells of a 96 well tissue culture plate (Costar #3595), cover, and place at 4 C overnight. Coat in triplicate or quadruplicate.
3) Invert plate and shake out coating solution. Pull off remaining coating solution from each well with a yellow tip pipetter.