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3H-Thymidine Uptake by Cultured Cells
(Source: 51bio)
LEVEL II
Materials
- Fibroblast cells in log phase growth
- Ca++, Mg++free-phosphate buffered saline (PBSA)
- 5% (w/v) Glutaraldehyde (GTA)
- 2% (w/v) Perchloric Acid (PCA)
- Subbed slides (coated with chrom alum gelatin) and Permount
- Nuclear Track Emulsion (Kodak or Ilford)
- Darkroom and chemicals for photographic processing
Dektol developer
Kodak Fixer
- Giemsa stain, graded series of alcohols, xylol