Bioprotocols

It's really difficult to keep human embryonic stem cells (hESCs) at undifferential status for long. Fortunately, we have moved forward at rat bone mesenchymal stem cell research, and I submit the abstract to a meetig.

Adipogenic Differentiation from Mesenchymal Stem Cell (MSCs) Involves PTHrP and PPAR-γ Pathways

Abstract

Background: The potential use of cell-based therapy is one of the most exciting advances in the field of translational medicine to treat various pathological conditions that have been untreatable up to now. Chronic lung disease is rapidly becoming the primary cause of death in the US. Currently, there is no intervention that can either arrest or cure evolving or established chronic lung diseases. Knowledge of the intrinsic molecular mechanisms that allow for resistance to inflammation and recruitment of stem cells for repair would allow for the engineering of such cells for effective treatment. MSCs are bone marrow non-haematopoietic stem cells that are multipotent and can differentiate into bone, cartilage and connective tissues. Moreover MSCs present many advantages as facilities to culture or to transform genetically. We have discovered that Parathyroid Hormone-related Protein (PTHrP) and PPAR-r drive the key homeostatic lung epithelial-mesenchymal interactions, resulting in the differentiation of alveolar interstitial lipofibroblasts that are essential for normal lung development and its repair following injury. In this paper, we try to isolate CD45 (-) CD73 (+) CD90 (+) MSCs from 6 weeks old rats and further induce such cells into adipogenic cells. Meanwhile, we try to confirm our hypothesis that PTHrP and PPAR-γ related pathways are involved in the MSCs differential procedure.

Methods: Femurs and tibias were aseptically harvested from 6 weeks old Wistar rats. Whole bone marrow plugs were obtained by flushing the bone marrow cavity with an 18-gauge needle set with a syringe filled with α-MEM medium. Second passage of adherent MSCs were

Tags: Cellular Biology

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