Bioprotocols

Protocols for DNA

1. Methylene Blue DNA staining protocol

2. Plasmid Protocols--(31-37)

3. Plasmid Protocols--(21-30)

4. Plasmid Protocol--(1-10)

5. Microsatellites Protocols

6. In-Situ hybridization to Embryonic Tissue Section

7. DNA ligation Protocol  

8. Colony Hybridization Protocols

9. Colony Hybridization  

10. DNA Isolation, Purification and Troubleshooting  

11. Deoxyribose Isolation from DNA Degrasion

12. Mitochondrial DNA Isolation

13. Genomic DNA Isolation from Specific Samples

14. Genome DNA Extraction

15. End Labeling Protocols (for DNA & RNA)

16. DNA FISH Protocol

17. PCR analysis of DNA: Embryo Genotyping Protocol

18. DNA Methods （3）

19. Collection of Materials for DNA Analysis

20. DNA Labeling Protocols

Protocols for RNA

1. IN VITRO TRANSCRIPTION OF RNA

2. SINGLE CELL mRNA AMPLIFICATION (REVERSE NORTHERN ANALYSIS)

3. Protocols for SiRNA

4. SELEX—in vitro selection

5. Nucleic Acids Research Methods (2)

6. RNA MicroArray Protocol

7. RNA Isolation

8. RNA FISH Protocols

9. shRNA Protocols

10.Reverse Transfection Protocols 

Protocols for PCR

1. PCR Based Molecular Cloning

2. Handbook for DNA isolation, RAPD-PCR and PCR-RFLP

3. Introduction to PCR

4. General PCR introduction

5. PCR Animations

6. PCR, RT PCR and Real Time PCR Tutorials

7. PCR Application Manual