Viral Protocols and Protocol Links
Creation and Use of Infectious Virus Vector
http://www.stewartlab.net/Protocols/Making_Virus_1...
(Stewart Lab, Washington University at St. Louis)
Extraction of Geminiviral DNA from a Highly Mucilaginous Plant Protocol
http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb18/R00-056.pdf
Protocol for the extraction of geminiviral DNA from a highly mucilaginous plant (Abelmoschus esculentus). Includes: Extraction; Alkali lysis; Cloning.
Generation of recombinant baculoviruses by co-transfection
http://www.its.caltech.edu/%7Ebjorker/Baculovirus-...
For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
Gradient Purification of AT2 Inactivated HIV-1 Protocol
http://ubik.microbiol.washington.edu/protocols/bl3/at2.htm
Protocol for gradient purification of AT2 inactivated HIV-1.
HIV-1 p24 Antigen Capture ELISA Protocol
http://ubik.microbiol.washington.edu/protocols/bl3/p24elisa.htm
Protocol for HIV-1 p24 antigen capture ELISA.
Information Resources for Adjuvants and Antibody Production
http://www.nal.usda.gov/awic/pubs/antibody/
Article explaining the function of adjuvants, providing guidelines for their use, particularly Freund's adjuvants, and introducing some alternatives that are commercially available.
Immunohistochemistry: In Situ Hybridization
http://www.immunoportal.com/
Includes details of immunoenzyme techniques, immunofluorescence and in situ hybridizations, with a photograph gallery and discussion board.
Purification of Bacteriophage lambda Arms: Centrifugation through Sucrose Density Gradients Protocol
http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3990
Protocol is used to prepare the arms of any bacteriophage {lambda} vector.
Purification of Bacteriophage lambda Particles by Isopycnic Centrifugation through CsCl Gradients
http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3968
Isopycnic centrifugation through CsCl gradients is used to prepare infectious bacteriophage {lambda} particles of the highest purity that are essentially free of contaminating bacterial nucleic acids.
Purification of Recombinant Adeno-Associated Virus (rAAV) and Parovirus Protocol
http://www.axis-shield.com/densityhome/optiprep/M04.pdf
Protocol for the purification of recombinant adeno-associated virus (rAAV) and parvovirus in pre-formed iodixanol gradients.
Retroviral infection of primary human keratinocytes (PHKs)
http://www.methodbook.net/cellcult/infectn.html
(Matt Lewis, Department of Pathology, University of Liverpool)
SDS-PAGE Sample Preparation of Adenovirus Infected Cells Protocol
http://www.bio.com/protocolstools/protocol.jhtml?id=p1752
Protocol for SDS-PAGE sample preparation of adenovirus infected cells.
http://openwetware.org/wiki/Keating:Experimental_Protocols:TEV_purification
Protocol for TEV purification.
Titration of Retrovirus Protocol
http://www.methodbook.net/virus/titratn.html
Protocol for the titration of retrovirus.
Viral Detection and Concentration in Food
http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf
Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods.
http://www.bio.com/protocolstools/protocol.jhtml?id=p617
Protocol describes the production of a dot blot membrane spotted with RNA isolated from virus particles.
Virus detection protocols for west nile virus in vertebrate and ...
http://www.ncbi.nlm.nih.gov/pubmed/12904372
Protocols to detect the presence of WNV have been refined since 1999 for ... Polymerase Chain Reaction/methods; RNA, Viral/isolation & purification ...