Page 1 of 3
Adapted from Sambrook, Fritsch, and Maniatis Molecular Cloning (1989)
MATERIALS:
Soln A (10% SDS)
Soln B (0.5N NaOH, 1.5M NaCl)
Soln C (0.5M Tris-Cl, 1.5M NaCl pH 7.4)
Soln D (2X SSC)
Nylon-backed Membrane (1), cut to size of 100mm petri dish
Whatman 3MM or other Blotting Paper (5)
15 x 150mm petri dish (4)