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PARAFFIN SECTIONS
Preparation of embryos (¾0 hr) for paraffin sections
Wash embryos off collection plates onto a nylon screen with 0.4% NaCl/0.3% Triton X-100. Rinse well to remove all food and yeast. Blot well to remove excess liquid.
Dechorionate embryos in freshly diluted 50% chlorox for 3' at room temperature with gentle agitation. Rinse with NaCl/Triton; blot well.
Transfer dechorionated embryos to either buffer A or B (depending on their age):
Buffer A (0-2, 1-3 hr embryos)
2.5 ml 8% paraformaldehyde (fresh)
2.0 ml 1X PBS
0.5 ml DMSO
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