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PCR analysis of DNA from Laser Microdissected (LM) Samples - Embryo Genotyping Protocol
Use sterile conditions through out protocol.
Sterilize the Arcturus heat block and amber colored tray with RNase Zap (Ambion, cat# AM9780) and rinse with Nuclease-free water. Blot with Kim wipes and set under hood to dry.
Set LM lab oven at 65°C. Once up to temperature, place sterile Arcturus heat block in oven with holes up.
Fit Heater/shaker with 500 µl tube holder, turn on and set to 95°C to preheat.