Page 1 of 13
End-labeling DNA fragments
End-labeled DNA fragments are used in a wide variety of
molecular biology experiments. Doubley end-labeled fragments are useful
as MW standards on Southern blots, as probes for filter-binding or gel
retardation experiments, or for visualizing small amounts of restricted
DNA. Singley end-labeled DNA is used for Maxam & Gilbert sequencing,
for S1 analysis, footprinting, primer extension, etc.
This method can also be used for 5' end-labeling RNA molecules.