DNA Nanoparticles Protocols
Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery
Weijun Li and Francis C. Szoka, Jr.1
Department of Biopharmaceutical Sciences and Pharmaceutical Chemistry, School of Pharmacy, University of California at San Francisco, California 94143-0446, USA
1Corresponding author ( This e-mail address is being protected from spambots. You need JavaScript enabled to view it )
INTRODUCTION
This protocol describes a stepwise procedure to prepare nucleic acids encapsulated in a polyethylene glycol (PEG)-shielded nanolipoparticle (NLP) that contain a bioresponsive lipid and ligand. This process provides several advantages for systemic gene delivery. The in vivo circulation time is extended. Also, low pH-sensitive lipids enhance DNA unpacking and endosomal escape. Finally, ligands inserted into the NLP surface can target gene delivery to specific tissues or cells in vivo.