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Home Immunology Immunohistochemistry: Retrievals

Immunohistochemistry: Retrievals

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The protocols for heat-induced epitope retrieval and enzymatic epitope retrieval.

Heat-Induced Epitope Retrieval

 

Decloaker

  1. Add 500ml distilled water to the pan of the Decloaker™.
  2. Place a full rack of slides in a Tissue Tek™ container containing 250ml of retrieval solution.
  3. Decloak for 5 minutes.
  4. Depressurize for 10 minutes.
  5. Remove pan top and cool for 10 minutes.
  6. Rinse in distilled water for 3 minutes. Repeat twice.