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The protocols for heat-induced epitope retrieval and enzymatic epitope retrieval.
Heat-Induced Epitope Retrieval
- Add 500ml distilled water to the pan of the Decloaker™.
- Place a full rack of slides in a Tissue Tek™ container containing 250ml of retrieval solution.
- Decloak for 5 minutes.
- Depressurize for 10 minutes.
- Remove pan top and cool for 10 minutes.
- Rinse in distilled water for 3 minutes. Repeat twice.