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1. Deparaffinize and rehydrate sections:
3 x 3´ Xylene
3 x 2´ 100% Ethanol
1 x 2´ 95%, 80%, 70% Ethanol (each)
1 x 5´ 1X PBS
2. Antigen retrieval methods:
Sodium Citrate Antigen Retrieval:
a. Place slides in a glass slide holder and fill in the rest of the rack with blank
slides (10 total) to ensure even heating.
b. Place rack in 600 ml of 10 mM Sodium Citrate (pH 6.0, 100 mM stock) in a
glass 2L beaker. Mark a line at the top of the liquid on the beaker.
c. Microwave for 20 minutes total, replacing evaporated water every 10 min.
d. Cool slides for 20 minutes in the beaker.
e. Wash 3 x 5´ in dd H2O, 1 x 5´ in 1X PBS.