Chromatin Immunoprecipitation (ChIP) Assay Protocols
What is ChIP?
The principle of ChIP is simple: the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognise a protein or protein modification of interest can be used to determine the relative abundance of that antigen at one or more locations in the genome.
ChIP consists of the following steps: 1. Isolation of total chromatin (which may require cross-linking to fix the antigen of interest to its chromatin binding site) 2. Fragmentation of the chromatin (to achieve resolution) 3. Immunoprecipitation of the resulting chromatin fragments 4. Analysis of the immunoprecipitated fraction to determine the level of a target DNA sequence (or sequences) relative to its abundance in the input chromatin. The final analysis is typically carried out using PCR or hybridisation-based techniques. A comprehensive review of the procedures and methodologies of ChIP can be found in Allis & Wu (2004).
Protocols—for Chip on Chip
Protocols used in ChIP-on-chip experiments. Listed here are collections of RenLab protocols as well as protocals requested from other labs. ...
www.chiponchip.org/protocol.html