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Purpose:
Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40% glycerol. It is lab policy to prepare a frozen stock of newly acquired or created strains for the archives as soon as possible.
Time required:
Cells are grown 6 hours to overnight; a total of less than 5 minutes bench time for each strain.
Procedure:
Day 1
- Inoculate a 15 ml culture tube containing 5 ml of LBM or LBM+antibiotic selective medium with a freshly grown isolated colony. Incubate at 37 degrees C until culture is in late log or stationary phase (usually 5 hours to overnight).