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This is a detailed troubleshooting tip for PCR.
Why do I have non-specific bands when I run my gel?
Causes：Template concentration is inappropriate
Measures：Use appropriate template concentrations. For a 50 μl
PCR reaction recommended concentrations are:
human genomic DNA= 0.1-1 μg; E.coli genomic
DNA = 10-100ng; lphage DNA= 0.5-2.5 ng;
plasmid DNA 10-100 ng
Causes：Damaged template DNA