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The PCR product may require purification first. Use "GenElute agarose spin column" (sigma 5-6500) if the product is in a gel or "QIAquick PCR purification kit" if it is liquid.
1- Equipment
§ heating block
§ spinning centrifuge
2- Reagents
§ Vector: Promega pGEM-T cloning kit
§ Competent cells: XL2-Blue (Stratagene 200150)
§ SOC medium
§ ß-mercaptoethanol (ß-MAE)
3- Other consumables
§ 0.3 µl sterile ??? tubes
§ DW