Bioprotocols

The In Situ PCR:

(Amplification and Detection in a Cellular Context)

Ernest F. Retzel, Katherine A. Staskus, Janet E. Embretson and Ashley T. Haase

Department of Microbiology, University of Minnesota, Minneapolis, MN 55455

Table of Contents

Introduction
Technical Aspects/Philosophy
Primer Design
Reaction Conditions
"Mechanics" of the Reaction
Methodology

A. Cell and Tissues.

1. Cultured Cells: Infection and Preparation.
2. Tissues: Pulmonary infection and preparation.

B. In Situ Amplification

1. Cultured Cells in Suspension.
2. Tissues on Slides.

C. Detection of Amplified DNA by in situ Hybridization

1. Solution amplification.
2. Tissue Sections.

References