Degenerate PCR
The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the ...
www.dartmouth.edu/~ambros/protocols/other/koelle/degenerate_PCR.html
Degenerate PCR.html (Michael Koelle)
by Michael Koelle. The identification of novel members of gene families by PCR using degenerate primers has been considered more of an art than a science, ...
www.med.yale.edu/mbb/koelle/protocols/protocol_degenerate_PCR.html
PCR Primer Design
However, I have used primers with as high as 256- and 1024-fold degeneracy for the successful amplification and subsequent direct sequencing of a wide range ...
www.mcb.uct.ac.za/pcroptim.htm
Degenerate PCR (NTNU Cell and Molecular Biology Group)
X - Services · Open Positions, Degenerate PCR Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. ...
http://boneslab.bio.ntnu.no/degpcrshortguide.htm
Designing degenerate PCR primers
The purpose of CODEHOP is to help in the design of degenerate PCR primers from protein sequence. Given a multiple alignment of a series of target proteins, ...
genomebiology.com/2000/1/1/reports/240
Nature Protocols: Quantitative multiplex degenerate PCR for human endogenous retrovirus expression profiling.
Last, the protocol below provides general rules for the design of MD-PCR applications. Once primers have been designed and optimized, the procedure can be ...
www.natureprotocols.com/2007/01/25/quantitative_multiplex_degener.php