Colony PCR protocol
Detailed protocol from the web site of the Department of Biology, University of Michigan, USA.
www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/colony_pcr.html
Colony PCR
Colony PCR. This protocol is designed to quickly screen for plasmid inserts directly from E. coli. colonies. The plasmid should be high copy number such as ...
www.csun.edu/~mls42367/Protocols/Colony%20PCR.pdf
Colony PCR Protocol
Colony PCR Protocol. 1. Pull out eight glycerol stock plates from the –80. o. C freezer and set on bench top to. thaw. Be sure to remove the foil seal ...
microarrays.berkeley.edu/file_download.php?fid=13
Colony PCR Protocol
Colony PCR Protocol. Overnight Culture 5.0µl. 10x PCR buffer 5.0µl. MgCl2 (25mM) 5.0µl. Forward primer (10µM) 0.2µl. Reverse primer (10µM) 0.2µl ...
www.unc.edu/~fconlon/Protocols/Colony%20PCR%20Protocol.doc
Colony PCR
Colony PCR Protocol contributed by Lynn Hancock 1. Choose a reasonable sized colony (2-3 mm in diameter) and resuspend 100 µl ddH2O. 2. For PCR, ...
www.enterococcus.ouhsc.edu/ColonyPCRProtocol.asp