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Home PCR Variants of PCR (1)

Variants of PCR (1)

From Wikipedia, the free encyclopedia

 This page assumes familiarity with the terms and components used in the Polymerase Chain Reaction (PCR) process.

The versatility of PCR has led to a large number of variants:

 Contents

Basic modifications

Pretreatments and extensions

 

Buffer and temperature modifications

Primer modifications

Polymerase modifications

Mechanism modifications

Isothermal amplification methods

Additional reading

References

Basic modifications

Often only a small modification needs to be made to the 'standard' PCR protocol to achieve a desired goal:

*   One of the first adjustments made to PCR was the amplification of more than one target in a single tube. Multiplex-PCR can involve up to a dozen pairs of primers acting independently. This modification might be used simply to avoid having to prepare many individual reactions, or could allow the simultaneous analysis of multiple targets in a sample that has only a single copy of a genome. In testing for genetic disease mutations, six or more amplifications might be combined. In the standard protocol for DNA Fingerprinting, the 13 targets assayed are often amplified in groups of 3 or 4. Multiplex Ligation-dependent Probe Amplification (or MLPA) permits multiple targets to be amplified using only a single pair or primers, avoiding the resolution limitations of multiplex PCR.



Last Updated ( Friday, 26 September 2008 05:24 )