Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is usually a biological tissue or a microbial culture. The various steps in the purification process may free the protein from a matrix that confines it, separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps exploit differences in protein size, physico-chemical properties and binding affinity.
1. ALKYL Aspartamide HIC Columns 2. Comparison of affinity tags for protein purification. 5. Green Fluorescent Protein Isolation (Gary J. Lindquester) 6. GST Fusion Protein Preparation 7. GST Fusion Protein Purification from Yeast 8. GST-Fusion Protein Purification 9. HeLa Cell Nuclei Preparation (John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN) 10. HILIC and SEC Columns PolySULFOETHYL and PolyHYDROXYETHYL Columns 11. Isolation of Protein from Tissue (Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University) 13. Making Conductivity Curves (Waters Lab) 14. PI-PLC Purification (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology) 15. Polyaspartic Acid Weak Cation Exchange Columns 16. PolyGLYCOPLEX Complex Carbohydrates Columns 17. PolySULFOETHYL Aspartamide Strong Cation Exchange Columns 18. PolyWAX LP Weak Anion Exchange Columns 19. Preparation of Tonsil Lysate (Springer Lab, Harvard University) 20. Protein Extraction (Frank Bottone Jr.) 21. Protein Extraction from Tissues (Reddy research laboratory, Neurological Sciences Institute, Oregon Health & Science University) 22. Protein Isolation using Trizol (Frank Bottone Jr.)
Protein Isolation Protocols (Lamond Lab, University of Dundee)