General Western Blot Protocol 1. Polyacrylamide gel electrophoresis and blotting a. Add an appropriate amount of electrophoresis sample buffer (1X = 125mM ...
http://www.alphagenix.com/phpkb/question.php?ID=1
Western Blotting and Immunostaining Protocol. Wash XCell box, blotting module and trays with soap and water before proceeding. Reagents:. Transfer buffer ...
http://structbio.vanderbilt.edu/chazin/wisdom/labpro/Western_Blotting.html
2) If you've never used an antibody for western blotting before, it is a good idea to dilute it, say 1:200, 1:500, and 1:1000, and compare the signals and ...
http://pingu.salk.edu/~sefton/Hyper_protocols/western.html
WESTERN BLOTTING USING ANTIBODIES AGAINST HISTONE PROTEINS
The following protocol refers to the Western blot detection of histone ... Top Tips for Successful Western blotting with our range of Histone antibodies. ...
www.abcam.com/ps/pdf/protocols/Histone%20western%20blotting.pdf