Periodic Acid Schiff (PAS) Glycogen Staining Protocol
Fixation: 10% formalin for 10 minutes.
Section: paraffin
sections at 6 micro meter.
Solutions and Reagents:
0.5 % Periodic Acid Solution:
Periodic
acid ------------------- 0.5 g
Distilled water ------------------100 ml
Schiff Reagent
Hematoxylin
Solution:
Procedure:
1. Deparaffinize and rehydrate.
2. Rinse in 0.5%
periodic acid solution for 10 minutes.
3. Rinse in distilled water.
4. Place in Schiff
reagent for 15 minutes (Sections become light pink color during this step).
5.
6. Counterstain in hematoxylin for 30 seconds.
7. Wash
in tap water for 5 minutes.
8. Dehydrate and mount with a coverslip.
Results:
Glycogen, mucin and some basement membranes --- red/purple
Background -----------------------------------------------
blue
PAS reactions ¨C Reaction intensities will vary; however, the following substances will be PAS-positive (magenta): glycogen, neutral mucosubstances, basement membranes, collagen fibers, fungal cell walls, and glycoproteins such as yolk vesicles and phospholipids.