Everything you must know for tissue culture

Cell Biology Laboratory Manual

Table of Contents


Chapter 1: The Microscope

Chapter 2: Histochemistry

Chapter 3: Cell Fractionation

Chapter 4: Electrophoresis

Chapter 5: Enzymes

Chapter 6: Membranes

Chapter 7: Microsomes

Chapter 8: Photosynthesis\Respiration

Chapter 9: Tubules\Filaments

Chapter 10: Chromosomes

Chapter 11: Cell Cycles

Chapter 12: Cell Cultures

Chapter 13: Differentiation

Chapter 14: Nucleic Acids

Chapter 15: The Central Dogma

Appendix A: Units and Measures

Appendix B: Statistics

Appendix C: Graphs

Appendix D: Computers

Appendix E: Image Analysis

Appendix F: CentrifugationAppendix G: Spectrophotometry

Appendix H: Radioactive tracers

Appendix I: Photography

Appendix J: Chemical Preparations

 

 

Reference: http://homepages.gac.edu/~cellab/contents.html

Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College,
St. Peter, MN 56082

 

 

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Protocols / Cellular Biology

General cell culture protocols

Chromosome karyotype
Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)
Cell cycle assay 
Migration assay
Stem cell & related protocols 
Apoptosis and related protocols 
Soft Agar Assay for Colony Formation
Aorta ring assay
GFP transfection 
Blood cell fractionation (white blood cell isolation) 
Endothelial isolation and culture protocols
Isolation and culture human brain microvessel endothelial cells
Gene transfection
Transformation protocols
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