What is apoptosis?
Apoptosis is a form of programmed cell death involving a serial of biochemical cascades including such proteins
as Bcl-2, Bax, Apaf-1 or apoptotic protease activating factor-1, caspases such as caspase-9, caspase-3, and caspase-7, as well as
proteins involved in digestion of proteins, degradation of DNA, and phagocytosis. Apoptosis is a normal cellular process and is essential
for the proper development and maintenance of the tissues and organs. Apoptosis is also necessary for the destruction of cells considered
a threat such as cells infected with viruses, cells with DNA damage, mutated cells, cancerous cells, and cells of the immune system
after they have fulfilled their function. The decision of a cell to undergo apoptosis depends upon the balance between positive signals
keeping the cell alive (eg, growth factors and interleukins such as IL-2) and negative signals that call for cellular suicide (eg,oxidative stress, DNA damage, improper protein folding, and specific molecules such as tumor necrosis factor alpha and beta (TNF-alpha
and TNF-beta), and the FAS ligand (FasL) that binds to the Fas receptor, or CD95).
Apoptosis (from Pomega)
Apoptosis vs. Necrosis 1.
Apoptosis vs. Necrosis 2.
Apoptosis vs. Necrosis
3.
Cell Death: Apoptosis and Necrosis
Protocols for Apoptosis
DNA Laddering:
1. Apoptosis assay using DNA
laddering.
2. Apoptosis assay using DNA laddering
3. Genome DNA Apoptosis Assay
4. An integrative procedure for apoptosis
identification and measurement
TUNEL
1. TUNEL for paraffin embedded sections
2. TUNEL Protocol
3. TUNEL Protocol
Using TACS TdT Reagents
DAPI staining of cells
1. DAPI staining
Apoptosis Assay by Annexin V Binding
1. Protocol ofApoptosis Assay by Annexin V Binding
Other Online Protocols for Apoptosis Assay (1) (2)
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