Gene Transfection
1.
Basic knowledge of gene transfection.
2.
Protocol 1. Gene transfection and its related issues. (A must read manual).
3.
Protocol 2. Cationic polymer transfection method.
4.Protocol 3.
Calcium phosphate precipitation
5.Protocol 4.
Calcium-phosphate-mediated transfection
6.Protocol 5.
Lipofectamine transfection protocol
7.
Protocol 6. Work with retrovirus vector.
8.
Protocol 7. Work with adenovirus.
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Protocols
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Cellular Biology
General cell culture protocols
Chromosome karyotype
Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)
Cell cycle assay
Migration assay
Stem cell & related protocols
Apoptosis and related protocols
Soft Agar Assay for Colony Formation
Aorta ring assay
GFP transfection
Blood cell fractionation (white blood cell isolation)
Endothelial isolation and culture protocols
Isolation and culture human brain microvessel endothelial cells
Gene transfection
Transformation protocols
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