A more flexible strategy allows the reversible regulation of transgene expression. The transgene is under the control of a responsive element (RE), which in turn is under the control of a transcription activator (TA).

 

The tetracycline regulated system (tet-system) is one of the most popular systems. In this system, transgenic expression of the GOI is under the control of a minimal promoter containing the tetracycline responsive element, tet operator, TetO. Two models of tet regulation exist depending on TA used. 1) tet-off: addition of tetracycline leads to downregulation of gene expression. 2) tet-on: addition of tetracycline leads to induction of gene expression.

 

 

 

 

References: 1. Regulated gene expression systems

 

                      2.Conditional and inducible transgene expression in mice through the conditional

                         use of Cre-mediated recombination and tetracycline induction

 

 

 

 

||WebHome | Home | Protocols | Bio Video | Resource | Medicine | Forum ||