DNA Isolation and Related Protocols
Contents
1. Dissolve 100ug DNA in 200ul distilled water.
2. Add MgCL2 and sodium acetate to the final concentration of 15mM and 10mM, respectively.
3. Incubate with 2 units DNAse I at room temperature for 15 minutes.
4. Add ammonium bicarbonate to the final concentration of 100mM, and then incubate with 2mU phosphodiesterase at 37oC in water bath for 2 hours.
5. Add 1 unit of alkaline phosphatase and incubated at 37oC water bath for another
1 hour.
6. Air dry the solution with degraded DNA. Derivalize produced deoxyribose to its aldonitrile
acetate form as usual for analysis
on GC/MS spectrometer.