(Modified by Ryoji Kojima, Takumi Yoshida and Jeffrey Randall, 8.20.2000)

 

1. Total RNA Isolation from cultured cells.

 

2. DNase Treatment of Total RNA 

 

3. Making the single strand cDNA probe.

 

4. Automated Slide Processor (ASP) Version for hybridization.

 

5. Washing microarrays in ASP.

 

6.Processing of Array slide

 

7. Pre-hybridization of the processed slides (NON-Automated version).

 

8. Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).

 

9. Preparation of Dendrimer Cy3 and Cy5.

 

10. Washing unbound probe from glass array (NON-Automated version).

 

11. Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).

 

12.Washing unbound dendrimer from glass array(NON-Automated version).

 

Microarray Dababases

 

Troubleshooting

 

Other Microarray Protocols (1)