DNA Library Construction
 
In biological engineering and combinatorial chemistry, it shows great promise in
discovering and creating novel materials which perform as artificial receptors or catalysts.
A property of the library is most important and progress had been made in
constructing biological library of peptide1,polysome2, phage3 and nucleic acid. Here are some
protocols that will be useful to your research work.
 
1.  Genomic library, cDNA library, YAC library, and screening of library.
 
2.  Standard microquantity cDNA libraries.
 
3.  cDNA library screening protocol
 
4.  cDNA library construction protocol.
 
5. Microsatellite library.
 
6.  Microsaellite library protocol
 
7.  Random shotgun library construction.
 
8. Methods for use with mTn-3xA/GFP  mutagenized library.
 
9.  Genomic library construction.
 
10.Plasmid library amplification.
 
11.PCR based ¦ËDNA library screening method
 
 
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Protocols / Molecular Biology

DNA isolation & related protocols  

DNA Purification (glass milk vs electroelution)
DNA and RNA sequencing
Nucleic acid methods (1)
Nucleic acid methods (2)
Isolation of DNA,RNA, and Protein simultaneously.
DNA mutation detection by SSCP 
Mouse genotyping by PCR
PCR,RT-PCR,Real time PCR etc.
Southern blot hybridization
Loss of Heterozygosity (LOH) 
Gene knockout protocol 
RNA Isolation and Purification 
Preparation of DNA and RNA probes
Northern blot hybridization  
SiRNA gene knockout
Western blot hybridization
Molecular cloning 
DNA protein interactions
Conditional gene transfection(Tet on/off)
Protein sequencing
Protein labeling techniques  
Subcellular fractionations  
Plasmid and its usefulness
DNA library construction
Microarray protocols.
Protein chips 

Methods for detecting protein phosphorylation

Protein methods
Molecular separation
Gene therapy for cancer
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