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Protein methods are the techniques used to study proteins.
There are genetic methods for studying proteins, methods for detecting proteins, methods for isolating and purifying
proteins and other methods for characterizing the structure and function of proteins, often requiring that the protein first be purified.
Genetic
methods
conceptual translation-
many proteins are never directly sequenced, but their sequence of amino acids is known by "conceptual translation" of a known mRNA
sequence. See Genetic code.
site-directed mutagenesis allows new variants of proteins to be produced and tested for how structural changes alter protein function.
insertion of protein tags such as the His-tag. See also: Green fluorescent protein.
evolutionary; analysis of sequence changes in different species using software such as BLAST.
Proteins that are involved in human diseases can be identified by matching alleles to disease and other phenotypes using methods such
as calculation of LOD scores.
Detecting proteins
microscopy, Protein immunostaining
immunoprecipitation, Protein immunoprecipitation
Western blot
Spectrophotometry
Enzyme assay
Protein purification
Protein Isolation
chromatography methods
Protein Extraction and Solubilization
Protein Concentration Determination Methods, Bradford protein assay
Concentrating Protein Solutions
Gel electrophoresis
Gel Electrophoresis Under denaturing conditions
Gel Electrophoresis Under non-denaturing conditions
2D Gel Electrophoresis
Electrofocusing
Protein structures
X-ray crystallography
Protein NMR
Hydrogen-deuterium exchange
Mass spectrometry
Molecular
dynamics
Protein structure
prediction
Protein sequencing
Protein structural alignment
Protein ontology
Peptide
mass fingerprinting
Ligand
binding assay
metabolic
labeling
heavy isotope labeling
radioactive isotope labeling
"Protein Methods", 2nd Edition by Daniel M. Bollag, Michael D. Rozycki and Stuart J. Edelstein (1996) Published by Wiley PblishersISBN 0-471-11837-0.