Bioprotocols

Your Lab's Handbook

Tuesday
Sep 07th
Text size
  • Increase font size
  • Default font size
  • Decrease font size
Home RNA PROTOCOL FOR SINGLE CELL mRNA AMPLIFICATION - Page 7

PROTOCOL FOR SINGLE CELL mRNA AMPLIFICATION - Page 7

E-mail Print PDF

References:

Springer Protocols: Abstract: Single-Cell Laser-Capture ...

To permit single-cell gene-expression profiling, the RNA amplification method has to be ... (2000) High-fidelity mRNA amplification for gene profiling. Nat. ...
www.springerprotocols.com/Abstract/doi/10.1385/1-59259-770-X:099

Single-Cell Antisense RNA Amplification and Microarray Analysis as ...

The use of single-cell mRNA amplification techniques such as the aRNA protocol is central to this line of investigation. Coupled with immunocytochemistry ...
sageke.sciencemag.org/cgi/content/full/2002/1/re1

One-Tube Protocol for Single-Cell Reverse Transcriptase-Polymerase Reaction.

tive enough for single-cell amplification and that im-. provement was needed. A multi-tube nested RT-PCR protocol amplified over-. expressed hKv2.1 mRNA ...
linkinghub.elsevier.com/retrieve/pii/S000326970295623X

An improved single-cell cDNA amplification method for efficient high-density oligonucleitide microarray analysis.

Therefore, single-cell mRNAs (~0.2 pg) could be amplified up to ~200 ng (~10 µg ..... the exponential amplification protocol offers better cost-performance. ...
www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1409679

Expression profiling from a single cell

Expression profiling from a single cell. The MessageBOOSTER™ Kit amplifies mRNA from total RNA of 1 - 50 cells (10 pg - 500 pg total RNA) and then produces ...
www.tebu-bio.com/file/thematic/50/335/

Nature Protocols: Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray.

We describe here a protocol for the representative amplification of global mRNAs from typical single mammalian cells to provide a template for high-density ...
www.natureprotocols.com/2007/05/24/global_singlecell_cdna_amplifi.php

 




 
 
 

Bo Blog Tags

Cellular Biology Genetics