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Preparation of material
- Prepare a maximum of 12 samples per chip.
- Maximum concentration recommended is 500ng/µl, 1000ng/µl is okay.
- Denature RNA 70°C 2min, cool on ice.
Cleaning, gel preparation (start 40min before experiment)
- take out filtered gel aliquot and fluorescent dye from fridge next door 30min ahead of time (1 gel aliquot tube enough for 2 chips)
- take out ladder from -80 freezer nextdoor (r145), column 5, nano white box / pico yellow
- vortex dye 10s and spin down
- mix one tube gel aliquot with 1µl of dye
- vortex, centrifuge 13000g +-20% for 10min (12000 rpm on Biofuge pico)
- wash electrodes 1min RNase ZAP, 2x30s water (pipette 500µl into any well, spreads from there)