Biotechniques

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Welcome to Biotechniques

Welcome to Biotechniques--Biologist's Community

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Biotechniques is a developing community website with a bio forum, videos, databases, pathways and bioprotocols. You are encouraged to register to get access to all the resource. On the other hand, you are honored to submit your protocols to this site to share with the community. Your name and your lab's name will be listed together with the protocols.

We can neither confirm all the source of the contents, nor the accuracy of the protocols subject to our limited hands. You'd better check the contents yourself before using. Please do feel free to let us know if any of the contents violated your copyright, and we will response properly.

Sincerely,

Peter L. Guo

This e-mail address is being protected from spambots. You need JavaScript enabled to view it  

at Los Angeles Biomedical  Institute.

 

Directory (1)

Protocols for DNA

1. Methylene Blue DNA staining protocol

2. Plasmid Protocols--(31-37)

3. Plasmid Protocols--(21-30)

4. Plasmid Protocol--(1-10)

5. Microsatellites Protocols

6. In-Situ hybridization to Embryonic Tissue Section

7. DNA ligation Protocol  

8. Colony Hybridization Protocols

9. Colony Hybridization  

10. DNA Isolation, Purification and Troubleshooting  

11. Deoxyribose Isolation from DNA Degrasion

12. Mitochondrial DNA Isolation

13. Genomic DNA Isolation from Specific Samples

14. Genome DNA Extraction

Protocols for RNA

1. IN VITRO TRANSCRIPTION OF RNA

2. SINGLE CELL mRNA AMPLIFICATION (REVERSE NORTHERN ANALYSIS)

3. Protocols for SiRNA

4. SELEX—in vitro selection

5. Nucleic Acids Research Methods (2)

6. RNA MicroArray Protocol

7. RNA Isolation

Protocols for PCR

1. PCR Based Molecular Cloning

2. Handbook for DNA isolation, RAPD-PCR and PCR-RFLP

3. Introduction to PCR

4. General PCR introduction

5. PCR Animations

6. PCR, RT PCR and Real Time PCR Tutorials

7. PCR Application Manual

8. PCR Elisa

9. PCR RFLP

10. PCR SSCP

11. Real Time PCR

12. RT-PCR Protocols

 

Archive (1)

1.General Lab Techniques

2. Lab security and basic techniques  

3. Advanced lab skills-(1)

4. Advanced lab skills-(2)

5. Molecular Biology

6. DNA isolation & related protocols  

7. DNA Purification (glass milk vs electroelution)

8. DNA and RNA sequencing

9. Nucleic acid methods (1)

10.Nucleic acid methods (2)

11.Isolation of DNA,RNA, and Protein simultaneously.

12.DNA mutation detection by SSCP 

13.Mouse genotyping by PCR

14.PCR,RT-PCR,Real time PCR etc.

15.Southern blot hybridization

16. Loss of Heterozygosity (LOH) 

17.Gene knockout protocol 

18.RNA Isolation and Purification 

19.Preparation of DNA and RNA probes

20.Northern blot hybridization  

21.SiRNA gene knockout

22.Western blot hybridization

23.Molecular cloning 

24.Conditional gene transfection(Tet on/off)

25.Protein sequencing

26.Protein labeling techniques  

27.Subcellular fractionations  

28.EMSA 

29.Methylation interference

30.Southwestern blotting

31.Filter binding protocols

32.Footpring protocols  

33.DNA (RNA) protein interactions

34.Plasmid and its usefulness

35.DNA library construction

36.Microarray protocols.

37. Protein chips 

38.Detecting of protein phosphorylation

39.Protein methods

40.Molecular separation

41.Gene therapy for cancer

42.X-ray crystallography

43.Metabolism and chromatographies

 



 

 


 

Directory (2)

13. Variants of PCR (2)

14. Variants of PCR (1)

15. AFLP PCR

16. Alu-PCR

17. Asymmetric PCR

18. Colony PCR Protocols

19. Competitive and Quantitative RT-PCR

20. Degenerate PCR

21. Differential Display PCR

22. In Situ PCR

23. Inverse PCR

24. Ligation Mediated Suppression PCR

25. Long PCR Protocols

26. Methylation Specific PCR

27. Multiplex PCR

28. Nested PCR

29. RACE PCR

30. RAPD PCR

31. Rep-PCR 

32. TAIL PCR

33. Touchdown PCR

34. Vectorette PCR

Protocols for Protein

1. Enzyme Assay（43-47）

2. Enzyme Assay（36-42）

3. Enzyme Assay（22-28）

4. Protocols for In Vitro Translation

5. Dot Blot Protocols

6. Selected Protocols of Protein Purification

7. Western Blotting Protocols (1)

Protocols for Cells

1. FACS for Bone marrow macrophages

2. Bone Marrow Macrophages

3. Metabolic Labeling of Cells with 35S

4. Protocols from the science advisory board

5. Introduction to Animal Cell Culture

6. Cell Transfection Protocols

 

Archive (2)

44.GC/MS Background

45.Glucose metabolism and its related protocols

46.PAS staining

47.Polysaccharide sequencing

48.Deoxyribose procedure

49.Ribose metabolism analysis

50.Lactate cleanup and derivative

51.Amino acids

52.Fatty acids

53.Lipid protocols

54.Cholesterol

55.Bile acids

56.Urea procedure

57.Choline Incorporation Assay

58.Isotope Ratio Mass Spectrometer (IRMS)

59.Liquid chromatography / mass spectrometer (LC/MS)

60.Proteomics

61.NMR protocols and tutorials

62.HPLC protocols

63.TLC

64.Molecular labeling

65.Immunology / immunohistochemistry

66.Preparing silanized (+plus) slides

67.Histological fixation

68.Microwave histology

69.Microscopy Techniques

70. Electron microscopy

71.HE staining 

72.Nucleic acid stain

73.Special cell & cell fraction stains

74.Antibody purification

75.Antibody storage and handling

76.Conjugation of monoclonal antibodies 

77.Antigen retrieval

78.Elisa

79.FRQs for histochemistry

80.Immunoperoxidase staining techniques


 

 

Directory (3)

7. Laser Capture Microdissection Protocols

8. Stem Cell Research Techniques and Protocols

9. XTT Cell Viability Assay Protocol

10. Apoptosis Assay Protocols

Protocols for Genetics

1. Genetic Analyses for DNA Protein Interactions

2. Comet Assay

3. Epigenetics Protocols

Protocols for Immunology and IHC

1. Grocott's Methenamine Silver (GMS) stain and troubleshooting

2. Elegans Immunohistochemistry

3. Cytokine Assay Protocols

4. Fluorescence in situ hybridization (FISH)

5. Radioimmunoassay--RIA Protocols

6. Competitive ELISA Protocols

7. Immunohistochemistry Stain for Frozen tissue

Protocols for Chromatography

1. Polysaccharide sequencing

2. Application Guides and Protocols (Nest Group)

3. HPLC Troubleshooting

4. Introduction to HPLC

Protocols for Model Organisms

1. Yeast Protocols

Protocols for Animal Experiment

1. Murine Models of Human Breast Cancer (B)

2. Chorioallantoic Membrane Vascular Assay

3. Guidelines for Techniques with Rodents (Duke University)

4. Tail Vein Injection Techniques

5. Animal Search Engines

Protocols for Basic Techniques

1. H & E Stain Troubleshooting

2. H and E Stain Protocols

3. Electrophoresis Protocols (11-26)

4. Lab. Safety in Life Science

 

Archive (3)

81.Immunofluorohistochemistry    

82.Confocal microscope technique 

83.Laser Capture Microdissection

84.Immunoprecipitation

85.ChIP assay

86.Hybridization in situ

87.Histotechnology--technical methods

88. Flow cytometry (FCM)

89.Kinase assay

90.Cellular Biology

91.General cell culture protocols

92.Chromosome karyotype

93.Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

94.Cell cycle assay 

95.Migration assay

96.Stem cell & related protocols 

97.Apoptosis and related protocols 

98.Soft Agar Assay for Colony Formation

99.Aorta ring assay

100.GFP transfection 

101.Gene transfection

102.Transformation protocols

103.Blood cell fractionation (white blood cell isolation) 

104.Endothelial isolation and culture

105.Isolation and culture human brain microvessel endothelial cells

106.Animal Experiments

107.Blood sampling from animals

108.Basic skills for animal experiments

109.Cancer xenograft animal models

110.Transgenic animal procedures

111.Transgenic cancer models

112.Animal search

113.Animal models for depression-like and anxiety-like behavior 

114.Genetics

115.Epigenetics protocols

116.SNPs

117.Genetic analysis

118.Mutagenesis

119.Model Organisms

120.C. Elegans

121.Yeast protocols and resource

122. Web links of bioprotocols (1)

123. Web links of bioprotocols (2)

 
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