Volume : 100 µL
Clone Number :
Aliases : Cdk 5 antibody; Cdk5 antibody; CDK5_HUMAN antibody; Cell division protein kinase 5 antibody; Crk6 antibody; Cyclin dependent kinase 5 antibody; Cyclin-dependent kinase 5 antibody; Protein kinase CDK5 splicing antibody; PSSALRE antibody; Serine threonine protein kinase PSSALRE antibody; Serine/threonine-protein kinase PSSALRE antibody; Tau protein kinase II catalytic subunit antibody; TPKII catalytic subunit antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q00535
Immunogen : SyntheVolumed non-phosphopeptide derived from Human CDK5 around the phosphorylation site of tyrosine 15 (G-T-Y(p)-G-T).
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC; WB : 1 : 500-1 : 3000, IHC : 1 : 50-1 : 100
Background : Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1 MEF2A, SIPA1L1 SH3GLB1 PXN, PAK1 MCAM/MUC18, SEPT5, SYN1 DNM1 AMPH, SYNJ1 CDK16, RAC1 RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1 p53/TP53, HDAC1 APEX1 PTK2/FAK1 huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Reg µLates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostim µLation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1 RAC1 RHOA, CDC42) or microtub µLe-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and mod µLates actin dynamics to reg µLate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1 and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1 AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), reg µLates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity; synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively reg µLating of JNK3/MAPK10 activity. Phosphorylation of p53/TP53 in response to genotoxic and oxidative stresses enhances its stabilization by preventing ubiquitin ligase-mediated proteasomal degradation, and induces transactivation of p53/TP53 target genes, thus reg µLating apoptosis. Phosphorylation of p35/CDK5R1 enhances its stabilization by preventing calpain-mediated proteolysis producing p25/CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal degradation. During aberrant cell-cycle activity and DNA damage, p25/CDK5 activity elicits cell-cycle activity and double-strand DNA breaks that precedes neuronal death by dereg µLating HDAC1. DNA damage triggered phosphorylation of huntingtin/HTT in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Phosphorylation of PXN reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Negative reg µLator of Wnt/beta-catenin signaling pathway. Activator of the GAIT (IFN-gamma-activated inhibitor of translation) pathway, which suppresses expression of a post-transcriptional reg µLon of proinflammatory genes in myeloid cells; phosphorylates the linker domain of glutamyl-prolyl tRNA synthetase (EPRS) in a IFN-gamma-dependent manner, the initial event in assembly of the GAIT complex. Phosphorylation of SH3GLB1 is required for autophagy induction in starved neurons. Phosphorylation of TONEBP/NFAT5 in response to osmotic stress mediates its rapid nuclear localization. MEF2 is inactivated by phosphorylation in nucleus in response to neurotoxin, thus leading to neuronal apoptosis. APEX1 AP-endodeoxyribonuclease is repressed by phosphorylation, res µLting in accum µLation of DNA damage and contributing to neuronal death. NOS3 phosphorylation down reg µLates NOS3-derived nitrite (NO) levels. SRC phosphorylation mediates its ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. May reg µLate endothelial cell migration and angiogenesis via the mod µLation of lamellipodia formation. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. The complex p35/CDK5 participates in the reg µLation of the circadian clock by mod µLating the function of CLOCK protein : phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and reg µLates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcell µLar distribution.
Meyerson M., EMBO J. 11 : 2909-2917(1992).
Meyerson M.; Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conj µgate : Non-conj µgated
Buffer : Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : CDK5
Research Areas : Neuroscience?Cancer;Cell biology;Metabolism